Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

A previously undescribed monopartite begomovirus was identified in Kampot province, Cambodia, Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of infections. The apparently full-length viral component cloned and sequenced following enrichment circular DNA by rolling-circle amplification restriction enzyme digestion. genome the virus 2737 nucleotides length (KP188831) exhibited an organization like that other begomoviruses, sharing highest nucleotide sequence similarity (87.7% identity) with ageratum (AM940137). satellite molecule amplified from total PCR amplification, using betasatellite-specific primer pair β01/β02. (1346 nt, KP188832) had structural characteristics those betasatellites associated begomoviruses shared (84.8% malvastrum betasatellite (MN205547). According to criteria established for species demarcation classification (family Geminiviridae) Tolecusatellitidae), respectively, isolate M. Cambodia is a novel begomovirus, which name “malvastrum virus” (MaYVCV) proposed, betasatellite, betasatellite” (MaYVKHB) proposed.